I.Z.S.L.E.R. - SCHEDA TECNICA DI PRODOTTO - POS.SC/101.005 REV. 01 Data emissione 21.11.2012 Pag. 1 di 1 Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna - Brescia CELL CULTURES CODE: CELL CULTURE: DESCRIPTION: SPECIES: SOURCE: BS TCL 213 SW 1353 Human humerus chondrosarcoma (*) Primates (Human) American Type Culture Collection, Manassas, Va (USA) DATE OF ARRIVAL: 1997 PASSAGE LEVEL No. AT ARRIVAL: 22 GROWING MEDIUM: Leibovitz's (L15) medium + fetal calf serum (10%) FREEZING MEDIUM: Culture medium + DMSO (10%) TOTAL CELL/ML: 3.0 x 10^6 TOTAL CELL/75CM2: 3.2 x 10^6 MORPHOLOGY: Fibroblastic-like ACTUAL PASSAGE LEVEL N.: SPLIT: 1:2 KARYOTYPE: 2n = 46 in 50 cells STERILITY TESTS: Free of bacteria and mycoplasma; not contaminated by: EBV, HBV, HCMV, HPV, HCV, HIV, HTLV-1, HTLV-2, HSV-1, HSV-2, HHV-6, HHV-8, Influenza type A VIRUS SENSIBILITY: Unknown BIOSAFETY LEVEL: 2 IDENTIFICATION NO.: APPLICATION: MONOCLONAL ANTIBODY: REFERENCES: NOTE: CONTROL OF SPECIES: ID ALZHEIMER: UPDATE: DEPARTMENT CODE: Unknown Not applicable See ATCC catalogue (*) This cell line will not be used for commercial purposes and it can't be distributed to third parties. A signed form is required Species confirmed by isoenzyme analysis (NP, G6PD, MD, MPI, AST and LD) Not applicable 02/03/2012 RSCIC 4917