Istituto Zooprofilattico Sperimentale della Lombardia e dell`Emilia

I.Z.S.L.E.R. - SCHEDA TECNICA DI PRODOTTO - POS.SC/101.005 REV. 01 Data emissione 21.11.2012 Pag. 1 di 1
Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna
- Brescia CELL CULTURES
CODE:
BS TCL 53
CELL CULTURE:
QT 35
DESCRIPTION:
Japanese quail tumor
SPECIES:
Poultry (Turkey)
ISOLATION AREA:
/
SOURCE:
Tumor Virology Laboratory, Gainesville (USA)
DATE OF ARRIVAL:
1982
PASSAGE LEVEL No. AT ARRIVAL: 61
GROWING MEDIUM:
Eagle's minimum essential medium in Earle's BSS +
fetal bovine serum (10%)
FREEZING MEDIUM:
Culture medium + DMSO (10%)
TOTAL CELL/ML:
3.0 x 10^6
TOTAL CELL/75CM2:
4.0 x 1^6
MORPHOLOGY:
Fibroblastic-like
ACTUAL PASSAGE LEVEL N.:
76
SPLIT:
1:2
KARYOTYPE:
2n = 78
STERILITY TESTS:
Free of bacteria and mycoplasma
VIRUS SENSIBILITY:
Avian sarcoma (B77) and Rous sarcoma viruses;
turkey herpesvirus (HVT); avian reticuloendotheliosis
virus; avian birnavirus; coronavirus; paramyxovirus;
poxvirus; reovirus and retrovirus
BIOSAFETY LEVEL:
1
IDENTIFICATION NO.:
APPLICATION:
MONOCLONAL ANTIBODY:
REFERENCES:
TISSUE/ORGAN:
NOTES:
CONTROL OF SPECIES:
ID ALZHEIMER:
UPDATE:
Virus cultivation
NOT APPLICABLE
Cell 11 : 95, 1977; Avian Dis. 25 : 839, 1981; Avian
Dis. 27 : 261, 1983; Avian Dis. 32 : 282, 1988
TUMOR
NO
Quail tissue derivation has been confirmed following
amplification of cell DNA and subsequent sequencing
of the 12S rRNA gene amplified.
NOT APPLICABLE
25/11/2015