I.Z.S.L.E.R. - SCHEDA TECNICA DI PRODOTTO - POS.SC/101.005 REV. 01 Data emissione 21.11.2012 Pag. 1 di 1 Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna - Brescia CELL CULTURES CODE: BS TCL 53 CELL CULTURE: QT 35 DESCRIPTION: Japanese quail tumor SPECIES: Poultry (Turkey) ISOLATION AREA: / SOURCE: Tumor Virology Laboratory, Gainesville (USA) DATE OF ARRIVAL: 1982 PASSAGE LEVEL No. AT ARRIVAL: 61 GROWING MEDIUM: Eagle's minimum essential medium in Earle's BSS + fetal bovine serum (10%) FREEZING MEDIUM: Culture medium + DMSO (10%) TOTAL CELL/ML: 3.0 x 10^6 TOTAL CELL/75CM2: 4.0 x 1^6 MORPHOLOGY: Fibroblastic-like ACTUAL PASSAGE LEVEL N.: 76 SPLIT: 1:2 KARYOTYPE: 2n = 78 STERILITY TESTS: Free of bacteria and mycoplasma VIRUS SENSIBILITY: Avian sarcoma (B77) and Rous sarcoma viruses; turkey herpesvirus (HVT); avian reticuloendotheliosis virus; avian birnavirus; coronavirus; paramyxovirus; poxvirus; reovirus and retrovirus BIOSAFETY LEVEL: 1 IDENTIFICATION NO.: APPLICATION: MONOCLONAL ANTIBODY: REFERENCES: TISSUE/ORGAN: NOTES: CONTROL OF SPECIES: ID ALZHEIMER: UPDATE: Virus cultivation NOT APPLICABLE Cell 11 : 95, 1977; Avian Dis. 25 : 839, 1981; Avian Dis. 27 : 261, 1983; Avian Dis. 32 : 282, 1988 TUMOR NO Quail tissue derivation has been confirmed following amplification of cell DNA and subsequent sequencing of the 12S rRNA gene amplified. NOT APPLICABLE 25/11/2015