IMMAGINI NASCOSTE NEL GENOMA (e musiche) SERGIO BARLATI 03 – DICEMBRE - 2013 IL DNA E’ CONTENUTO NEI CROMOSOMI ED E’ COSTITUITO DA 4 MOLECOLE, IDENTIFICATE DALLE LETTERE A-T-C-G, LEGATE IN SEQUENZE RAPPRESENTATE DA MILIONI DI LETTERE CROMOSOMI IN CELLULA FEMMINILE CARIOTIPO: RICOSTRUZIONE DELLE COPPIE DI CREMOSOMI 2003 COMPLETATO IL SEQUENZIAMENTO DEL GENOMA UMANO HGSC : DNA da molti donatori CELERA: DNA da 5 soggetti IN GRIGIO LE ZONE NON SEQUENZIATE 2007: IL PRIMO GENOMA PERSONALE di Craig Venter - Fondatore di Celera (in 4 anni, al costo:75 milioni EURO) 2013: GENOMA PERSONALE (in pochi giorni, al costo: 5-6000 EURO) (BGI- ILLUMINA) SB Genoma umano Formato da 2 x 3,000,000,000 basi di DNA, localizzati in 22 paia di cromosomi (CHR) oltre al CHR X e CHR Y (24 CHR) Codifica per 20,318 geni codificanti proteine e per 22,560 “geni” codificanti RNA. Solo circa 1% del Genoma è di sequenze che codificano proteine e circa il 2%, complessivamente, che codifica “geni”. Circa 2,900,000,000 basi sono presenti nel Genoma con altre funzioni (note solo in parte). CHR Geni Numero basi 1 2.968 245.203.898 2 2.288 243.315.028 3 2.032 199.411.731 4 1.297 191.610.523 5 1.643 180.967.295 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 X Y 1.963 1.443 1.127 1.299 1.440 2.093 1.652 748 1.098 1.122 1.098 1.576 766 1.454 927 303 288 1.184 231 170.740.541 158.431.299 145.908.738 134.505.819 135.480.874 134.978.784 133.464.434 114.151.656 105.311.216 100.114.055 89.995.999 81.691.216 77.753.510 63.790.860 63.644.868 46.976.537 49.476.972 152.634.166 50.961.097 IMPORTANZA DEL DNA NON CODIFICANTE E’ più variabile, fra le diverse specie di vertebrati, rispetto al DNA codificante (es. Uomo/pesce palla). Vi sono sequenze non codificanti mantenute conservate in diverse specie di Vertebrati anche se non hanno associata una funzione nota. Il DNA non codificante è meno sensibile alle mutazioni di singole basi ma è conservato nelle singole specie animali e le caratterizza. Possibile rilevanza del DNA non codificante nel definire specie diverse e nella programmazione associata allo sviluppo embrionale e al differenziamento cellulare. Si sa ancora troppo poco del DNA non codificante che è uno dei più importanti temi di studio. Necessarie anche nuove strategie di analisi …..idee… CATENA DI DNA SEQUENZA DI DNA 3 MILIARDI DI NUCLETIDI: 200 VOLUMI DI 1000 PAGINE CTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTCTGAAAGTGGACCTATCAGCAGGATGTGGGTGGGAGCAGATTAGAGAATAAAAGCAGACTGCCTGAGCCAGCAGTGGCAACCCAATGGGGTCCCTTTCCATAC TGTGGAAGCTTCGTTCTTTCACTCTTTGCAATAAATCTTGCTATTGCTCACTCTTTGGGTCCACACTGCCTTTATGAGCTGTGACACTCACCGCAAAGGTCTGCAGCTTCACTCCTGAGCCAGTGAGACCACAACCCCACCA GAAAGAAGAAACTCAGAACACATCTGAACATCAGAAGAAACAAACTCCGGACGCGCCACCTTTAAGAACTGTAACACTCACCGCGAGGTTCCGCGTCTTCATTCTTGAAGTCAGTGAGACCAAGAACCCACCAATTCCAGA CACACTAGGACCCTGAGACAACCCCTAGAAGAGCACCTGGTTGATAACCCAGTTCCCATCTGGGATTTAGGGGACCTGGACAGCCCGGAAAATGAGCTCCTCATCTCTAACCCAGTTCCCCTGTGGGGATTTAGGGGACC AGGGACAGCCCGTTGCATGAGCCCCTGGACTCTAACCCAGTTCCCTTCTGGAATTTAGGGGCCCTGGGACAGCCCTGTACATGAGCTCCTGGTCTGTAACACAGTTCCCCTGTGGGGATTTAGGGACTTGGGCCTTCTGT CTTTGGGATCTACTCTCTATGGGCCACACAGATATGTCTTCCAACTTCCCTACACAGGGGGGACTTCAAAGAGTGCCTTGAGCTGATCTGGTGATTGCTTTTTTGTACTGTTATTTATCTTATTCTTTTCATTGTGAGGTACT GATGCAAACACTTTGTACGAAAAGGTCTTTCTCATCTCGGGAGTCCCCGTCTATTTGTCCCGGTCCCTGTTAACCCAGTCCCCGACAGGAGCCCCTTCTGCACCTTGAGCTCTCACCACTCACCGTCCATCCAGCCCCAGCT CTGCCTGCAACCCACCCATCCCTGGGACTCGGGCCTCCCCTCTCTAGTGGTCTGGTCATCAGGCCAGGGGCACGTGGAAGAAGCTATCGTGGCAAAGGGAGCAGTCATATCCCCAAAATCTGTGGTTGGTTTACCACCAC CATGGAAACCCCAGGGTGGGACTCTAGTTTCAGGTTGGAGCTGAGCCCTGTCGGGAATGAGCTTTCCCCAGCTATGGCTTCTTGGGGCCCCTGTGCCCTGAGCTGTGTCTCCCAGCATCGGGTCCCCACCATGCATATGG CCCACTCAGGCACAGTGCCGCGATGGCTGCATGCGTGAGGGGGGCCTGGGCCCAGGGCTGGGAGTCCTTTGTGTCTCATGGCCATGATTGTCCTTCCGAGTATGATATGGTGGCCAATTTCTTTTATTCTGTCGTTCAGA GTGAGTAAATGATGTAGAGTTCATGCAGAAAAAAATACAACAAAAACCAAGGGAACATAGAATTGGAAAACGCGTCACAGCAATGAGTTAAATAGGTAACAAATTTCATCATTTGAAGAAAGACTTAGAGTGCCAAAAGTG CCTCTTAAGTCTCCTTTAAAAAGTAGCAAAATTCATCCCTGAAGAAGCATCTTGGCCTTTTTCATGTACTCAGAGTGCTGGTGAAGAACAAAGATTGCTGAAACATTATGTACCTAACAGCGTTACAGGGTGTAGATAACAC ACTGGAAAACCTGGTCGTTACAGTGGACATATTCCAGGAAGTCCTTGCCTGAGGTTTTCCAAGTTATGGAATTGCTTGAGATTGGAAGAGGCGATGGAGGGTACAACTGTAATGCCCAACCTCATTTTTGCTAACCCTGTT TTTAGACTCTCCCTTTCCTTCAATCACCTAGCCTTCTTTCCACCTGAAAGGACTCTCCCTTAACTGAGAGAACCGGACAGACTCCATCTTGGCTCTTTCACTGGCAGCCCCTTCCTCAAAGACTTAACTCGTGCAAGCTGACT CCCAGGACATCCGAGAATGCAATTAACTGACAACCTACTGTGGCGAGCTACATCCGCAGTCCCCAGGAATTCGTCCGATTGATAACGCCCAATTACCCGCGTCTATCACCTTGTAATAGTCTTAAAGCACCTGCACCTGGA ACTGTTTACTTTCCTGTAACCATTTATCCTTTTAACATTTTGCCTGATTTACTTATGTAAAATTCTTTTAACTAGACCGCCACTCCCCTTTCTAAACAAAAGTATAAAAGAAAATCTAGCCCCTTCTTTGGGACTGAGACAATT TTGAGGTTAACGCAGGGTGCCTGTAATCCTAAGGGAGGAGACCGCCACTTCTGCTGCCCTTCCCTTCCCCACACCCCCTTCTCTAGTTTATGAAACAGGGAAAAAGGGAGAAAGCAAAAAGATAAAAAAAACAGAAGTAA GATAAATAGCTAGACGACCTTGGCAGCACCACCCGGCACTGGTGGTTAAAATAATAATAATAATAATATTAACCCCTGACCTAAACTACTTGTGTTATCTGTAAATTCCAGACACTGTATGAGGAAGCCCTGCAAAACTTTC TGTTCTGTTATCTGATGCGTGTAGCCCCCAGTCACGTTCCGATGCTTGCTCGATCTATCACGACCCTTTCAAGTGAACCCCTTAGAGTCGTAAACCCTTAAAAGGGCCAGGAATTTCGTTTTCGGGGAGCTCGGCTCTTCAG GCCCAAGTAAACCTGCCGTATCTCACCTGAGACCAACCCCCAACTACAAAACTCAACCTGGAATTTTCCCAGGACCAAACCCATCTATATTCTGTAACCCGAAACCTCAAAGCCTAACCCTAACCCTAACCCCTACAGTTGA GGTCCCCCCGCCCCTGTGGTTCCAGCTCAAGACAACCTGCCCCTCCGTGGGTTTGCAGGCCCTCTGGTGGGGGTGGGAGCTGGGGGCCACATACAGCTCTCTGAGCTTAAGCCATTTTCTTCCTTCATTCCTTCCTTCCTC CCTCCCTTCCCTTCCTTCCTCCCTCCCTCCCTCCTTCCCTCCCTCCCTTTTTTTTTTCAGGGTCTTGCTCTGTCACCCAGGCTGGAGTGCAGTGGCATGATCACAGCTGACTGCAGCCTCGGACTCCCAGGCTCAAGTTATCT TCCCTCCTCAGCCTCCAGAGTAGGTGGGACTACAGGAGTGTGCCATCGCACCCAGCGAATTTCTTAATTTTTATTTTGTAGAGATGAGGCCTCTCGATATTGTCCAGGCTTGGAAGTAGTTCTTGAAATTCAAGAGGATCTT GAAGTTCTGACCTCCTGTCAATATCCCTTCCCCTCACCTTGACCCTCCCATTCTGCCCCACCTGTCAGGATCACAAGGACCCCCAGATCAGCAGATGGGAACCGGACCAAAAAGAGAAATAGTGCTGTCCCGCCCATAAGT ACCACCCCCAGACTCCCTGCTCCACCCTCTGGACCACAGGGAGGCCCCATGCTCCATCCCTGAAAACCACCCCCAGACCCCCTGCTCCTCCCCACGGGACCGCCCCCAGATCCACTGTTCCTGTCCTCCGGACCACACCGG ACAGCTCCTTCCCTCGGCGCCATCCCCAGACCCCAGCTCCTCCCCTCAGGATCATCCCCAGACCCCCGCTCCTCCCATCAGGACCGCCCCCAGAACCCCCTGCTCCTCCCCACAGGACCACCCCCAGACCCCCGCTCCTCC CCTAAGGACCACCCACAGACCCCCACGCTTCCCCTCGGGACCACCCCCAGACCCCCGCTCCTCCCCTCAGGACCACCCCCAGATCCACTGTTCCTGTCCTCCGGACCACACCAGACAGCTCCTTCCCTCGGCGCCATCCCC AGACCCCCACGCTTCCCCTCGGGACCACCCCCAGACCCCCGCTCCTGCCCTCGGGACCACGCCCAGACCCCCTGCTCCTCCCCACGAAACCACGCCCAGTAACCCCCCTCTTCTCCCCTCAGGACCACCCCCAGACCCCCG CTCCTCCCCTGGGAACCACCCCCAGACCCCCTGCTCCTCCCCACGAAACCACCCCCAGACTCCCTGCTCCTGTCCTCCGGACCACGCGACTGCTCCTTCCCTCAGCGCCACCCCCAGACTGCCGCTCCTCCTGTCGGGACC CCCTGAGGCTTTCTCCACCCGGAGTGCGGGGTAGGGAGCAGACGGAGAGTGACGGAGGGTGACGGAGAGTGACGAAAGTAGACGATGTCTGACGGAGAAGAGCCGAGCGGAGCTGAAGGGCGGCGGAGAGTGACGGA AAGTGGCGAGAATTGACGGAAAGTGACGGGGACTGACGGACAGTGACGAAGAGTCACGAAATTTATCAGAGGGCGACAAAGAGGAAAGCGAAATGGTGAGATGCAGCCGGCCGAGCCTAATCGGAGATGACGGAAAGT GACGGAGAGGAACGAGGAGTAAAGAGGGGTGACGAAAAGAGCCGAAGCTGGTGGAGGCGAAGAACTGAGTGAGGGAAGATAGCCGAGATTAGCGGGTGGGCTGCAGCCGGGGCAGTCGCCCGAATGGGCGGGACCCC ACGGAGTTAGCGAGAGGATGCGAACAGCGGCCAGCCGGGCAGCACGCGAGCGAGGGAGGGCGTGGAGGGCCGTGGGTCCGCCTGCACTGAGGCAGGCATGCGTGGCACCGAGGTGACCCGGGTGGGAGGTGCACCG CCGCCCCCTGGCAGTCTCTCCGCGGAGCCCAAGCCCGTCTTCTCCGCCCCTTTGCAGACCTCGGCGCCCAGCCTGGCCCCTGACGCCCACCCGCGGCCCCACCCAGCGCCCGGGCCCACGAGGCCGAGGAGCGGCGGAG ACTAACGGCCCCTGGACCCCAGGCAGCACATGGCCCAGAGCATCCCAGCCCAGTGGAGGGCGGCACATGGCGGGAGGGCGGGAGTCCGTGTCCACTCATGGCCGGGGAGGGGAGGGCAAGTTCTGGTGGCTGGGGAG GCCTAGAGCATCACAGCCCAGTGGAGGGCTGCACATGGCAGGGGAGGGGAGAGCAAGAGTGTGCGGGCGAGGGAGGAGAGGGCAAGACTGCGTGTCCGCTCCAGTCTCTCTTCCTCATCTTATAAAGCCACGAGTCCC ATGACTGGGGACCCAACCTAATAACATTATCTAATCCTAATTGCCTCCCAAAGGCCATATCTCCAATCAGGACATGAATTCGGGGATTAAATTGCCAACACATGGCTGGGCGCGATGGCTTGTGCTTTTTTTTTTTTTTTTTT TGAGACGGAGTCTCGCTCTGTCGCCCAGGCTGGAGTGCAGTGGCGCTATCTCGGCTCACTGCAAGCTCTGCCTCCCGGGTTCACGCCATTCTCCTGCCTCAGCCTCCTGAGTAGCTGGGACTACAGGCGCCCGCCACCAC GCCCAGGTAATTTTTTTTTTTTTTTTTTTGTATTTCTTTGTAGAGACGGGGTTTCACCATGTTAGCCAGGATGGTCTCGATCTCCTGACCTCGTGATCCACCCATCTCGGTCTCCCAAAGTGCTAGGATTGCAGGCCTGAGC CACCGCACCCAGCTGCCTTGTGCTTTTAATCCCAGCACTTTCAGAGGCCAAGGCAGGCGATCACCTGAGGTCAGGAGTTCAAGACCAGCCTGGCCAACATGGTGAAACCCCATCTCTAATACAAATACAAAAAAAAAACAA AAAACGTTAGCCAGGAATGAGGCCCGGTGCTTGTAATCCTAAGGAAGGAGACCACCACTCCTCCTGCTGCCCTTCCCTTCCCCACACCGCTTCCTTAGTTTATAAAACAGGGAAAAAGGGAGAAAGCAAAAAGCTTAAAAA AAAAAAAAAAAAAACAGAAGTAAGATAAATAGCTAGATGATCTTGGCAGCACCACCCGACCCTGGTGGTTAAAATAATAATAATAATAATATTAACCCCTGACCTAAACTACTTGTGTTATCTGTAAATTCCAGACACTGTA TGAGGAAGCCCTGCAAAACTTTCTGTTCTGTTATCTGATGCGTGTAGCCCCCAGTCACGTTCCCCATGCTTCCTCGATCTATCACAACCCTTTCACGTAAACCCCTTAGAGTTGTAAACCCTTAAAAGGGCCAGGAATTTCG TTTTCGGGGAGCTTGGCTCTTCAGGCGCAAGTGTGCCGATGCTCCTGGCAGAGTAAAGCCCTTCCTTCTTTAACCCAGTGTCTGAGGAATTTTGTCTGCGGCTTGTCCTGCCACAATGCCAGCTACCAGGAGGCTGAGGT GTGAGAATCGCTCGAACCTGGGAGGCAGAGGTTGCAGCGAGCCGAGATCCCACGATTGCACTCCAGCCTGGGTGATAGATGACAGAGCAAGACACCATGTCAAAAAGAAAAGAAAAAAAATTGCCAACACATGAAATCTG GAGAACACATTCAAGCAATAGCACCATATAAATCTGTACATACCTTGCAAACACTGTAAACCTGCCGCATCTCACCTGAGACCAACCCCCAACCGCAAAACCCAGACCTGGAATTTTCCCAGGACCAAACCCATCTATATTC TGTAACCCGAAATCTCAAAGCCTAACCCTAACCCTAACCCCGACAGTTCAGGTCCCCCCGTCCCCGTAGCTCCAGCTCAAGACAACCTGCCCCTCCGTGGGTTTGCAGGCCCTTTGGTGGGGGTGGGAGCTGGGGGCCAC ACACAGCTCTCTGAGCTTAAGCCATTTCCTTCCTTCCTTCATTCCTTCCTTCCTCCCTCCCTCCCTCCTTCCCTCCCTCCCTTTTTTTTTTCAGGGTCTTGCTCTGTCACCCAGGCTGGAGTGCAGTGGCATGATCACAGCTG ACTGCAGCCTCGGACTCCCAAGCTCAAGCGATCTTCCCGCCTCAGCCTCCAGAGTAGGTGGGACTACAGGAGTGTGCCATCGCACGTGGCGAATTTCTTAATTTTTATTTTGTAGAGATGAGGCCTCCCGATATTGTCCAG GCTTGGAAGTAGTTCTTGAAATTCAAGAGGATCTGGAAGTTCTGGTCTCCTGTCAATATCCCTTTCTAAGATGAAATGTAGAATCCTCTAATCACCCCAGGATACCTTTTCCAGCTATATTACCCTTTCCTTTCAGCCGACTA GAGTGGAAGCTCAGCAAAGAACGGATCTATGCCTGCACACAGGCAGCTTTGAACTCACTGAGATCTATAGAAGAATTTTTTAAAATTTGCCAGAATGAAAAAGAAGTATCCTCCAGTGAGCCTCAAGGACAGCCAGGGAA GGCAGCTACTGGGACCTGCCACTGGCCAGGATTGAGAGAGTCTGAGGTGTCCATTCTCCTATGGATGGGTCACACATCAGAACTGAACCACAAACCCTTTCCTGCTCCCTTGGGACCAGGCTGCCACTCCTTCCTTCCCCT CACCTTGACCCTCCCATTCGGCCCCACCTGTCAGGATCACAAGGACCCCCAGATCAGCAGATGGGAACCGGACCAAAAAGAGAAATAGCGCTGTCCCGCCCAAAAGTACCAACCCCAGACCCCCTGCTCCACCCTCCGAA CCACAGGGAGACCCCATGCTCCACCCCTGAAAACCACATGCAGACCCCCGCTCCTCCTCTTAGGGCCATCCCCAGACCCCCCCTCCTACCCACGGGACCACACCCAGAACCCCCAGCTCCTCCCCTCGGGACCACCCCAGA CCCCCGCTCCTCCCCTCGGGACCACCCCCAGACCCCCGCTCCTCCCCTCGGGACCACCCCAGACCCCCGCTCCTCCCCTCGGGACCACCCCAGACCCCCTGCTCCTCCTCTTAGGACCATCCCCAGACCCCCCCTCCTACC CACGGGACCACACCCAGAACCCCCAGCTCCTCCCCTCGGGACCACCCCAGACCCCCGCTCCTCCCCTCGGGACCACCCCAGACCCCCGCTCCTCCCCTCGGGACCACCCCAGACCCCCGCTCCTCCCCTCGGGACCACCC CCAGACCCCCTGCTCCTCCTCTTAGGACCATCCCCAGACCCCCCCTCCTACCCACGGGACCACACCCAGAACCCCCAGCTCCTCCCCTCGGGACCACCCCCAGACCCCCTGCTCCTCCTCTTAGGACCACCCCAGACCCCC GCTCCTCCCCTCGGGACCACCCCAGACCCCCGCTCCTCCCCTCGGGACCACCCCAGACCCCCGCTCCTCCCCTCGGGACCACCCCCGACCCCCTGCTCCTCCTCTTAGGACCATCCCCAGACCCCCCCTCCTACCCACGGG ACCACACCCAGAACCCCCAGCTCCTCCCCTCGGGACCACCCCAGACCCCCGCTCCTCCCCTCGGGACCACCCCAGACCCCCGCTCCTCCCCTCGGGACCACCCCAGACCCCCGCTCCTCCCCTCGGGACCACCCCCAGAC CCCCTGCTCCTCCTCTTAGGACCATCCCCAGACCCCCCCTCCTACCCACGGGACCACACCCAGAACCCCCAGCTCCTCCCCTCGGGACCACCCCAGACCCCCGCTCCTCCCCTCGGGACCACCCCAGACCCCCGCTCCTCC CCTCGGGACCACCCCAGACCCCCGCTCCTCCCCTCGGGACCACCCCCAGAACCCCCGCTCCTCCCCTCGGGACCACCCCAGACCCCCGCTCCTCCCCTCGGGACCACCCCAGACCCCCGCTCCTCCCCTCGGGACCACCC CCAGACCCCCTGCTCCTCCTCTTAGGACCATCCCCAGACCCCCCCTCCTACCCACGGGACCACCCCCAGACCCCCCGCTCCTCCCCTCGGGACCACCTCAGACCCCCGCTCCTCCCCTCGGGACCACCCCAGACCCCCGCT CCTCCCCTCGGGACCACCCCAGACCCCCTGCTCCCATTTTCCAGACCACACCAGACGGCTTCTTCCCTCGGCACCACCCCCAGACTGCCGCTCCTCCTATCGGGACCTCCTCGGGGCCATCTCCAGACCCTCCCCCCGCTC CACCCCTCGGGTCCCTCTCGGGACCACCCTCAGACCCCCGCTCCTTCCCTCGGGATCACCTCGAGACCTCCCGCTCACCACCATCATTCAGGCCGGGTAGTGGGGAGAGGCCTCTCAGGAGGGACCAGGCCACCCTCCCA chrX - da 57621731 a 57630804: 9073 bp SVILUPPATO UN SOFTWARE PER TRASFORMARE IL GENOMA IN IMMAGINI chrX - da 57621731 a 58597343 975.613 bp A:VERDE T: ROSSO C: BLEU G: NERO INTERO GENOMA IN CIRCA 3000 PAGINE (SCHERMATE PC) SYMBOL COLOUR DNA COLOUR * Brevetto Europeo dell’Università di Brescia N.° 06115244.3. GENE: SMCY (41.00bp) CROMOSOMA Y DA: 20.200.000 A: 21.000.000 = 800.000bp CERCANDO NEL GENOMA (NON CODIFICANTE) E ABBINANDO DIVERSI COLORI SI POSSONO OTTENERE IMMAGINI CHE, OLTRE AD AVERE UN POSSIBILE VALORE «ESTETICO» (ASTRATTO) , HANNO ASSOCIATA UN’INFORMAZIONE. SEGUONO ALCUNI ESEMPI CHR2: DA 1.977.753 A 1.980.000 bps CHR2: DA 91.682.006 A 91.744.222 bps CHR3: DA 90.523.300 A 90.585.025 bps CHR4: DA 48.928.063 A 48.990.105 bps DA CHR 2 DA CHR17 DA CHR19 DA CHRs: XeY SEQUENZE ALTAMENTE VARIABILI COINVOLTE NELLA PLASTICITA’ DEL DNA DNA DINAMICI LE IMMAGINE OTTENUTE POSSONO ESSERE STAMPATE SU LASTRE DI VETRO PER OTTENERE QUELLI CHE HO DEFINITO: “VETRI PARLANTI” IMMAGINI ASTRATTE CHE HANNO ASSOCIATA UN’INFORMAZIONE (SPESSO «FUNZIONE» DA DEFINIRE) I VETRI PARLANTI I “VETRI PARLANTI” PRENDONO VITA DALLA LUCE DEL SOLE (CON IL SOLE IL DNA VIVE) “LE ALI DEL DNA” OPERA VINCITRICE, PER IL TEMA PITTURA, DEL I° CONCORSO D’ARTE DELL’Ist.Sup.Sanità. “ IL VOLO DI PEGASO” 2008/09 dal Cromosoma X dalla posizione: 61.598.923 a 61.729.14 Sergio Barlati FOTO DEL VETRO I° FESTIVAL RECYCLART MOSTRA “VETRI PARLANTI” - CANONICA DEL DUOMO CASTIGLIONE DELLE STIVIERE (MN) 2009 http://www.recyclart.it/2009/home.php?section=arte&sub=dna II° FESTIVAL RECYCLART “LE PORTE DELLA CONOSCENZA” - SALA ESPOSIZIONI CASTIGLIONE DELLE STIVIERE (MN) 2010 (TRASFORMAZIONE DI TESTI IN IMMAGINI – VEDI PIEGHEVOLI) IL DECALOGO DEL CERVELLO LA «TRINITA’» BIOLOGICA IL DECALOGO DEL DNA http://www.recyclart.it/home.php?section=arte&sub=dna PROGETTO PER EXPO 2015 LA «CATTEDRALE» DEL DNA PROPONENTE: Sergio Barlati UNIVERSITA’ DI BRESCIA IL PROGETTO HA AVUTO: BASSA PRIORITA’ DALLE IMMAGINI ALLA MUSICA AD OGNI LETTERA O SIMBOLO DA SEQUENZE DI: DNA, PROTEINE O TESTO, SI ABBINA UNA NOTA SPECIFICA CON CARATTERISTICHE SCELTE A PIACERE: STRUMENTI, OTTAVE, DURATA SEQUENZA DI DNA CHE CODIFICA PER IL GENE DEL “GLUTAMATE RECEPTOR 1” ATGCAGCACATTTTTGCCTTCTTCTGCACCGGTTTCCTAGGCGCGGTAGTAGGTGCCAATTTCCCCAACAATATCCAGATCGGGGGAT TATTTCCAAACCAGCAGTCACAGGAACATGCTGCTTTTAGATTTGCTTTGTCGCAACTCACAGAGCCCCCGAAGCTGCTCCCCCAGAT TGATATTGTGAACATCAGCGACAGCTTTGAGATGACCTATAGATTCTGTTCCCAGTTCTCCAAAGGAGTCTATGCCATCTTTGGGTTTT ATGAACGTAGGACTGTCAACATGCTGACCTCCTTTTGTGGGGCCCTCCACGTCTGCTTCATTACGCCGAGCTTTCCCGTTGATACATCC AATCAGTTTGTCCTTCAGCTGCGCCCTGAACTGCAGGATGCCCTCATCAGCATCATTGACCATTACAAGTGGCAGAAATTTGTCTACAT TTATGATGCCGACCGGGGCTTATCCGTCCTGCAGAAAGTCCTGGATACAGCTGCTGAGAAGAACTGGCAGGTGACAGCAGTCAACATT TTGACAACCACAGAGGAGGGATACCGGATGCTCTTTCAGGACCTGGAGAAGAAAAAGGAGCGGCTGGTGGTGGTGGACTGTGAATCA GAACGCCTCAATGCTATCTTGGGCCAGATTATAAAGCTAGAGAAGAATGGCATCGGCTACCACTACATTCTTGCAAATCTGGGCTTCA TGGACATTGACTTAAACAAATTCAAGGAGAGTGGCGCCAATGTGACAGGTTTCCAGCTGGTGAACTACACAGACACTATTCCGGCCAA GATCATGCAGCAGTGGAAGAATAGTGATGCTCGAGACCACACACGGGTGGACTGGAAGAGACCCAAGTACACCTCTGCGCTCACCTA CGATGGGGTGAAGGTGATGGCTGAGGCTTTCCAGAGCCTGCGGAGGCAGAGAATTGATATATCTCGCCGGGGGAATGCTGGGGATTG TCTGGCTAACCCAGCTGTTCCCTGGGGCCAAGGGATCGACATCCAGAGAGCTCTGCAGCAGGTGCGATTTGAAGGTTTAACAGGAAA CGTGCAGTTTAATGAGAAAGGACGCCGGACCAACTACACGCTCCACGTGATTGAAATGAAACATGACGGCATCCGAAAGATTGGTTAC TGGAATGAAGATGATAAGTTTGTCCCTGCAGCCACCGATGCCCAAGCTGGGGGCGATAATTCAAGTGTTCAGAACAGAACATACATCG TCACAACAATCCTAGAAGATCCTTATGTGATGCTCAAGAAGAACGCCAATCAGTTTGAGGGCAATGACCGTTACGAGGGCTACTGTGT AGAGCTGGCGGCAGAGATTGCCAAGCACGTGGGCTACTCCTACCGTCTGGAGATTGTCAGTGATGGAAAATACGGAGCCCGAGACCC TGACACGAAGGCCTGGAATGGCATGGTGGGAGAGCTGGTCTATGGAAGAGCAGATGTGGCTGTGGCTCCCTTAACTATCACTTTGGTC CGGGAAGAAGTTATAGATTTCTCCAAACCATTTATGAGTTTGGGGATCTCCATCATGATTAAAAAACCACAGAAATCCAAGCCGGGTG TCTTCTCCTTCCTTGATCCTTTGGCTTATGAGATTTGGATGTGCATTGTTTTTGCCTACATTGGAGTGAGTGTTGTCCTCTTCCTGGTCA GCCGCTTCAGTCCCTATGAATGGCACAGTGAAGAGTTTGAGGAAGGACGGGACCAGACAACCAGTGACCAGTCCAATGAGTTTGGGA TATTCAACAGTTTGTGGTTCTCCCTGGGAGCCTTCATGCAGCAAGGATGTGACATTTCTCCCAGGTCCCTGTCTGGTCGCATCGTTGGT GGCGTCTGGTGGTTCTTCACCTTAATCATCATCTCCTCATATACAGCCAATCTGGCCGCCTTCCTGACCGTGGAGAGGATGGTGTCTC CCATTGAGAGTGCAGAGGACCTAGCGAAGCAGACAGAAATTGCCTACGGGACGCTGGAAGCAGGATCTACTAAGGAGTTCTTCAGGA GGTCTAAAATTGCTGTGTTTGAGAAGATGTGGACATACATGAAGTCAGCAGAGCCATCAGTTTTTGTGCGGACCACAGAGGAGGGGAT GATTCGAGTGAGGAAATCCAAAGGCAAATATGCCTACCTCCTGGAGTCCACCATGAATGAGTACATTGAGCAGCGGAAACCCTGTGAC ACCATGAAGGTGGGAGGTAACTTGGATTCCAAAGGCTATGGCATTGCAACACCCAAGGGGTCTGCCCTGAGAAATCCAGTAAACCTG GCAGTGTTAAAACTGAACGAGCAGGGGCTTTTGGACAAATTGAAAAACAAATGGTGGTACGACAAGGGCGAGTGCGGCAGCGGGGGA GGTGATTCCAAGGACAAGACAAGCGCTCTGAGCCTCAGCAATGTGGCAGGCGTGTTCTACATCCTGATCGGAGGACTTGGACTAGCC ATGCTGGTTGCCTTAATCGAGTTCTGCTACAAATCCCGTAGTGAATCCAAGCGGATGAAGGGTTTTTGTTTGATCCCACAGCAATCCAT CAACGAAGCCATACGGACATCGACCCTCCCCCGCAACAGCGGGGCAGGAGCCAGCAGCGGCGGCAGTGGAGAGAATGGTCGGGTGG TCAGCCATGACTTCCCCAAGTCCATGCAATCGATTCCTTGCATGAGCCACAGTTCAGGGATGCCCTTGGGAGCCACGGGATTGTAA CODICE UTILIZZATO: 2 BASI del DNA (BP) (CORRISPONDENZE BP - NOTE (FAST) BP NOTE AA AC AG AT CA CC CG CT GA GC GG GT TC TG TT :DO : RE : MI : FA : SOL : LA : SI : DO : RE : MI : FA : SOL : SI : DO : DO OTT. TEMPO : 4 : 1/16 : 4 : 1/16 : 4 : 1/8 : 4 : 1/8 : 4 : 1/16 : 4 : 1/16 : 4 : 1/8 : 4 : 1/8 : 4 : 1/16 : 4 : 1/16 : 4 : 1/8 : 4 : 1/8 : 4 : 1/16 : 4 : 1/8 : 4 : 1/8 TAIKO DRUM CODICE UTILIZZATO: 2 BASI del DNA (BP) (CORRISPONDENZE BP - NOTE (SLOW) BP NOTE OTT. TEMPO AA AC AG AT CA CC CG CT GA GC GG GT TA TC TG TT : DO : RE : MI : FA : SOL : LA : SI : DO : RE : MI : FA : SOL : LA : SI : DO : DO :2 :2 :3 :3 :3 :3 :2 :2 :3 :3 :3 :3 :2 :3 :3 :2 : 1/4 : 1/4 : 1/8 : 1/8 : 1/4 : 1/4 : 1/8 : 1/4 : 1/4 : 1/4 : 1/8 : 1/4 : 1/4 : 1/4 : 1/8 : 1/4 LEAD3 (Organo - Calliope) Un grazie al Prof. Danieli per l’invito e un grazie sentito a Tutti Voi. Un ricordo a Frederick SANGER, Padre dell’Era Genomica, morto a 95 anni, il 19 Novembre scorso. «UN APPLAUSO X SANGER» GRAZIE Sergio Barlati