Nucleotide sequence of a cucumber mosaic virus

Nucleic Acids Research, 1992, Vol. 20, No. 24 6733
© 1992 Oxford University Press
Nucleotide sequence of a cucumber mosaic virus satellite
RNA associated with a tomato top stunting
Francesco Grieco, Fabrizio Cillo1, Loredana Barbarossa and Donato Gallitelli*
Dipartimento di Protezione delle Piante dalle Malattie, Universita di Bari and Centra di Studio sui virus
e le virosi delle colture mediterranee del CNR, 70126 Bari, and 1Tecnogen SCpA, 81015 Piana di
Monte Verna (CE), Italy
EMBL accession no. X69136
Received November 23, 1992; Accepted November 25, 1992
A strain of cucumber mosaic cucumovirus (CMV) belonging to
the WT subgroup has been isolated in Apulia (Southern Italy)
from tomato plants showing a severe shortening of internodes
of terminal shoots leading to a bushy appearance. The disease
was clearly distinguishable from other CMV-induced disorders
in tomato Italian crops like fruit necrosis, lethal necrosis and
shoestring-fem leaf (1). Virus preparations purified from naturally
infected plants contained CMV genomic RNAs 1 —4 and a
satellite RNA named TTS-CARNA 5 (CARNA 5 for CMVassociated RNA 5). The virus, for which the name of CMVTTS was adopted, was cultured in Rutgers tomato and purified
by a standardized procedure (2). We report here the nucleotide
sequence of its satellite RNA (Figure 1). Nucleotide sequence
of TTS-CARNA5 was determined using the dideoxy-chain
termination method on both strands of three different pUC-cloned
cDNA templates, and by direct sequencing on RNA template (3).
When compared with other CMV satellite RNAs (1, Bl, B2,
D, E, G, I17N, R, S, WL1, WL2, F, K8) (4, 5, 6) the following
nucleotides were found to be uniques for TTS-CARNA5: T38
(vs C), C 3 9 (vs. T), A269 (vs G). The 3' half of TTS-CARNA
5 does not contain the 'necrogenic consensus' reported for other
CMV satellite RNAs (4). Preliminary results strongly suggest
that TTS-CARNA5 is involved in causing tomato top stunting
by a modulation of symptoms induced by CMV helper strain.
ACKNOWLEDGEMENTS
This work was supported by a grant of MURST (art. 65
D.P.R.382/80 quota 40%) 'Genetica del virus del mosaico del
cetriolo e del suo RNA satellite in isolati da pomodoro'. Grateful
* To whom correspondence should be addressed
thanks are due to Dr P.Iosco, Metapontum Agrobios, Metaponto
(MT), Italy, for preparing the synthetic oligonucleotides used in
this study.
REFERENCES
1.
2.
3.
4.
5.
6.
Crescenzi.A. et al. (1992) Plant Disease 76, (in press).
Lot,H. et al. (1972) Ann. PhytopathoL 4, 2 5 - 3 8 .
Meshi.T. et al. (1983) FEBS Lett. 162, 282285.
Devic.M. etal., (1990)7. Gen. Virol. 71, 1443-1449.
Hidaka.S. et al. (1990) J. Gen. Virol. 71, 439-442.
Fraile A. et al. (1990) Nucleic Adds Res. 18, 4593.
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GUUUUGUUUC UUAGAGAAUU GCGUGGAGCG GUUAUAUUCA CGUGAGGAUC UAUCACUCGG
110
120
70
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CGGUGUGGGU UACCUCCCUG CUACGGCGGG UUGAGUUGAC GCACCUGCGA CUGGGGACCG
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CUGGCCUGCG AGCUAUGUCC GCUACIKUCA GCACUACGCA CUCAUUUGAG CCCCCGCUCA
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240
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GUUUGCUAGC AAAACCCGGC ACGUGGUUUG CCGUUACCAC GGAACUUCGA AAGAAACACU
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CUGUUAGGUG GUAUCGUGGA UGACGCACAC AGGGAGAGGC UAAAACCUAU ACGGUCAUGC
310
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UGAUCUCCGU GAAUGUCUAC ACAUUCCUCU ACAGGACCC
Figure 1. Complete nucleotide sequence of TTS-CARNA5.