DETECTION AND GENOTYPING OF HUMAN PAPILLOMA VIRUS

27° Congresso Nazionale SIMeL
TORINO 9-10 Ottobre 2013
DETECTION AND GENOTYPING OF
HUMAN PAPILLOMA VIRUS (HPV)
IN ORAL MUCOSA AND TONSIL:
CASE REPORT
ANALISI CLINICHE
MATER DEI
A. L. Gambardella, S. Marino, A. Rosolia
Laboratorio “Analisi Cliniche Mater Dei”, Pagani, (SA)
Aim of the Study
Human papillomavirus (HPV) is the most common sexually transmitted infection and is
the necessary cause of cervical cancer. Over 200 genotypes of papillomaviruses infect
the skin and mucosal surfaces. The majority of them have affinity to grow on skin, oral
lesions, genitals, anal and larynx. The potentially oncogenic HPV has been associated
with oral squamous cell carcinoma. Some evidence has linked them to orogenital contact
with the transmission of papillomavirus from the genital zone to the oral cavity.
The purpose of this investigation was to study the presence of HPV-DNA in oral cavity of
a woman which represents our case report.
Methods
This study researched the HPV DNA extracted from the oral cavity of a
patient with oral lesions and injury of the tonsil. Two regions were sampled
in the oral cavity; the first one was the buccal mucosa and the second one
was the tonsil.
Detection of Human papillomavirus was realized with extraction of DNA,
PCR protocol and reverse dot-blot with commercial kits (Innogenetics,
Gent, BE).
Model of HPV showing the
arrangement of capsid proteins
Schematic view of genotyping tests for the
identification of HPV genotypes.
Results
The analysis conducted on patient showed the presence of HPV-DNA. The typing of the main Papilloma Virus revealed
the presence of 2 different genotype as follows: HPV-56 on buccal mucosa and HPV 51 on tonsil. Although the 2 viral
genotype were different, they were both high oncogenic risk.
Conclusions
Previous studies have either investigated the relationship of HPV with oral cancer or the prevalence of HPV on the oral
cavity.
We have observed differences by the anatomic region of the oral cavity sampled, particularly, we have found 2 different
genotypes in 2 anatomical sites of the same woman.
This topic is of great importance, since the most attention is focused on understanding HPV infection to improve strategies
to prevent infection and disease in women.
References
1- Sánchez-Vargas LO, Díaz-Hernández C, Martinez-Martinez A. Detection of Human Papilloma Virus (HPV) in oral mucosa
of women with cervical lesions and their relation to oral sex practices. Infect Agent Cancer. 2010 Dec 4;5(1):25.
2- Ibieta BR, Lizano M, Fras-Mendivil M, Barrera JL, Carrillo A, Ma Ruz-Godoy L, Mohar A: Human papilloma virus in oral
squamous cell carcinoma in a Mexican population. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2005, 99:311-315.
3-Sugiyama M, Bhawal UK, Dohmen T, Ono S, Miyauchi M, Ishikawa T: Detection of human papillomavirus-16 and HPV18 DNA in normal, dysplastic, and malignant oral epithelium. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2003,
95:594-600.
“Analisi Cliniche Mater Dei” - Pagani (SA) - Tel. (+39) 081 916526 - Fax 081 5156121 Email: [email protected]